According to the gene sequence, design and screen amplification primers that specifically bind to specific genotypes. The gene primers are not used to label biotin group, digoxigenyl group, and FITC respectively. When there is a gene specifically binding to the primer in the sample, amplify The reaction occurs and a large number of amplified fragments with biotin (digoxigenin) and FITC binding are produced. The amplified product is combined with the colloidal gold coated with FITC antibody to form a conjugate. The biotin-labeled product is connected to the T1 line. Combined with color development, the digoxigenin-labeled product is combined with T2 line to develop color, and the individual genotype is judged based on the color development result. Lateral flow test strips can be tested in a convenient, instrument-free manner, and may be a good choice in some environments.

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